Sample handling

관리자 2021-10-26 15:00:06
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Quickly concentrates up to 15 mL of biological sample without valuable sample loss

  • Concentrate 15 mL sample volumes to 0.5 mL in 30 to 60 minutes.
  • High recoveries, typically greater than 90%.
  • Low protein-binding Omega™ membrane and polypropylene housing minimize losses due to non-specific binding.
  • Versatile. Omega membrane available in a variety of MWCOs color coded for easy identification.
  • Built-in deadstop prevents spinning to dryness.
  • Heat-sealed membrane design prevents retentate leakage into the filtrate receiver.
 


 
  • Concentrate and desalt proteins, DNA, and RNA.
  • Buffer exchange or salt removal of chromatography eluates and gradient fractions.
  • Recover proteins or other molecules from cell culture supernatants.
  • Clarify tissue homogenates.
  • Concentrate viruses.
  • Wash and concentrate latex suspensions.
     
    Materials of Construction
    Filter Media: Omega membrane (low protein-binding, modified polyethersulfone on polyethylene substrate)
    Sample Reservoir, Filtrate Receiver, and Concentrate Cup: Polypropylene
    Paddle (without Membrane) and Cap: Polyethylene
    Effective Filtration Area
    10 cm2

    Dimensions**
    Outside Diameter: 29 mm (1.1 in.)
    Overall Length (fully assembled with cap): 10.9 cm (4.3 in.)
    **Fits 50 mL tube adapters.
    Minimum Tube Diameter Adapters
    2.9 cm
    Capacities
    Maximum Sample Volume: 15 mL
    Final Concentrate Volume: 0.5 - 1.5 mL depending on rotor used
    Filtrate Receiver Volume: 15 mL
    Concentrate Cup Volume: 1.5 mL
    Hold-up Volume (membrane/support): 0.03 mL
    Operating Temperature Range
    0 - 40 °C (32 - 104 °F)
    pH Range
    1 - 14

    Maximum Centrifugal Force
    5,000 x g

    Centrifuge
    A fixed-angle or swinging bucket rotor is required that accepts 50 mL tubes and is capable of 1,000 to 5,000 x g

    Sanitization
    Provided non-sterile; may be sanitized by filtering 70% ethanol through the device prior to use
     
    Macrosep® Device 30K MWCO Concentration Rates
     
    Swinging Bucket Rotor, 1 mg/mL BSA
     
     
    Fixed-angle 45° Rotor, 1 mg/mL BSA
     
     
     

Part Number Description Pkg
Macrosep Centrifugal Devices with Omega Membrane
OD001C36 1K, yellow 6/pkg
OD001C37 1K, yellow 24/pkg
OD001C38 1K, yellow 100/pkg
OD003C36 3K, gray 6/pkg
OD003C37 3K, gray 24/pkg
OD003C38 3K, gray 100/pkg
OD010C36 10K, blue 6/pkg
OD010C37 10K, blue 24/pkg
OD010C38 10K, blue 100/pkg
OD030C36 30K, red 6/pkg
OD030C37 30K, red 24/pkg
OD030C38 30K, red 100/pkg
OD050C36 50K, green 6/pkg
OD050C37 50K, green 24/pkg
OD050C38 50K, green 100/pkg
OD100C36 100K, clear 6/pkg
OD100C37 100K, clear 24/pkg
OD100C38 100K, clear 100/pkg
OD300C36 300K, orange 6/pkg
OD300C37 300K, orange 24/pkg
OD300C38 300K, orange 100/pkg
OD990C36 1000K, purple 6/pkg
OD990C37 1000K, purple 24/pkg
OD990C38 1000K, purple 100/pkg
PLUS